"In situ" changes in enzyme activity during Neurospora conidial germination

The Guanine Nucleotide Exchange Factor RIC8 Regulates Conidial Germination through Gα Proteins in Neurospora crassa

Heterotrimeric G protein signaling is essential for normal hyphal growth in the filamentous fungus Neurospora crassa. We have previously demonstrated that the non-receptor guanine nucleotide exchange factor RIC8 acts upstream of the Gα proteins GNA-1 and GNA-3 to regulate hyphal extension. Here we demonstrate that regulation of hyphal extension results at least in part, from an important role i...

Neurospora crassa Hyphal Fusion in Pathway during Conidial Germination and Role of a Mitogen-Activated Protein Kinase

Changes in Enzyme Activity during Differentiation

The patterns of alanine dehydrogenase, glutamate dehydrogenase and malate dehydrogenase activity were studied during the normal vegetative cell cycle and during the processes of gametic differentiation and dedifferentiation in synchronized cultures of C h l a m y d o m o n a s reinhardtii. During all three phases of growth and differentiation the synthesis of DNA was also measured. During gamet...

Changes in the Permeability of Ascospores of Neurospora Tetrasperma during Germination

The respiration and germination of activated ascospores of Neurospora tetrasperma have been shown to be almost completely inhibited by concentrations of ethylene diaminetetraacetic acid (EDTA) as low as 0.0035 M. In contrast, however, dormant ascospores are insensitive to this chelating agent. At any time up to about 150 minutes after activation Ca(++) or Mg(++) can completely reverse this toxi...

Alternation in Erythrocyte Enzyme Antioxidant Activity during Blood Storage

Background: Blood is permanently exposed to oxidation stress and therefore has a high antioxidants capacity. Many different factors increasing the demand for the antioxidant capacity can be observed in the stored blood of donors. Consequently, damage to erythrocytes by free radicals may occur. So it is useful to control the alternation of anti-oxidant enzymes in stored blood at different days o...